Reeve Carney (Peter Parker/Spider-Man), Jennifer Damiano (Mary Jane Watson), America Olivo (u/s Arachne), Patrick Page (Norman Osborn/Green Goblin), Michael Mulheren (J. Jonah Jameson), Ken Marks (Uncle Ben/Buttons), Isabel Keating (Aunt May/Maxie/Mrs. Gribrock), Jeb Brown (MJ's Father/Stokes), Laura Beth Wells (Emily Osborn/Marbles), Matt Caplan (Flash/Bud), Dwayne Clark (Robertson/Boyle), Luther Creek (Travis/Kong), Gerald Avery (Swarm), Collin Baja (Carnage), Emmanuel Brown (Electro)