Jason Alexander (The Narrator), Keala Settle (Dr. Frank-N-Furter), Patrick Stump (Dr. Frank-N-Furter), Peppermint (Dr. Frank-N-Furter), Rumer Willis (Dr. Frank-N-Furter), Tim Curry (Dr. Frank-N-Furter), Barry Bostwick (Brad Majors), Wilmer Valderrama (Brad Majors), Colleen Ballinger (Janet Weiss), Marissa Jaret Winokur (Janet Weiss), Lance Bass (Rocky), Rosario Dawson (Magenta/Usherette), Nell Campbell (Columbia), David Arquette (Eddie/Dr Scott), Jenna Ushkowitz (Phantom #1), Josh Gad (Phantom #1), Karen Olivo (Phantom #1)